WRI Webinar: Duong Nguyen, SARDI & UWA
Dec 9, 10:00 am - 11:00 am
This webinar series showcases the excellent science our members, affiliates and collaborators are undertaking across the agriculture, food and wine sectors. Expand your network and find new collaborators!
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Duong Nguyen – Application of modern trait mapping methods to explore the genetic control of plant vigour in chickpea (Cicer arietinum L.)
SARDI, University of Western Australia
WHEN: Thursday 9th December 2021, 10am
Abstract: Vigour is a complex trait controlled by quantitative trait loci and plays a critical role in plant growth and adaptation to biotic and abiotic stress conditions. Plant vigour has been identified as high priority by breeding programmes in pulse crops. However, the molecular mechanisms controlling vigour in pulses remain unclear. Here, genomic data from a recombinant inbred line (RIL) population (Rupali/Genesis836) and multiple diversity panels were used to identify QTL and sequence-level haplotypes associated with vigour and vigour-related traits in different Australian environments. Major vigour QTL were found on chickpea chromosomes (Ca) 1, 3, and 4. Subsequently, the major QTL on Ca4, Ca4_Vqtl, was delimited to 104.4 kb, using recombinant and association analysis in 15 different heterozygous inbred families derived from the Rupali/Genesis836 RILs. Phenotypic and molecular genetic analysis revealed the potential role of a gene encoding a transcriptional regulator, Ca4_TIFY4B, in determining leaf and seed size in chickpea. Additionally, candidate genes underlying other QTL were proposed and validated for their association with the control of vigour across chickpea panels using KASP markers. These include myo -inositol monophosphatase (CaIMP) on Ca1 and a Flowering Time locus (FT) gene cluster (FTa1-FTa2-FTc) on Ca3. The KASP markers developed and validated from this work will provide breeders with robust molecular tools for selection for not only vigour but also seed size, a key trait attracting a price premium for chickpea.
Duong is a researcher from Field Crops Research Institute, Vietnam. His main research interest is quantitative genetics in plants. Duong is an UWA PhD student but externally enrolled at South Australian Research and Development Institute (SARDI), Adelaide. At SARDI, Duong is conducting research with the Molecular Genetics group under supervision of Dr Tim Sutton (SARDI and UoA) and Dr Julie Hayes (UoA). His PhD thesis aims to explore the genetic control of plant vigour in chickpea and assess the impact on yield in Australian environments. This research was supported by an Australian Government Research Training Program (RTP) Scholarship, UWA Fees Offset, and an Underwood PhD completion scholarship.