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CSIRO Waite Campus Seminar: Pat Corena
Jul 16, 4:00 pm
The following seminar will take place at 4:00pm on Tuesday 16th July in the CSIRO Wine Innovation West, Upstairs Seminar Room
Gene editing in grapevine using CRISPR/Cas9
Pat Corena | CSIRO Agriculture & Food
Genome editing is a rapidly expanding field that allows for specific gene modification. RNA guided nuclease systems such as CRISPR/Cas9 has become more widely used, the advantage being the use of a single nuclease CAS9 that can target any sequence containing a required protospacer adjacent motif (PAM). A gene editing vector that works in grapevine has been constructed which also allows easy substitution of sgRNAs. Best vector and guide RNA design, delivery systems and mutation screening methods have been determined. This study provides valuable information on how CRISPR/Cas9 works in grapevine.
ABOUT THE SPEAKER
Pat Corena has been working for CSIRO for 24 years as a Research Projects Officer. She started her science career by completing a Bachelor of Science Degree with Honours in Genetics at the University of Adelaide in 1992. During this time she won a 2 year scholarship in Italy at the Mario Negri Sud Centre of Pharmacology Research, Santa Maria Imbaro, Chieti, researching human clotting factors. From there she moved back to Adelaide to Flinders University where she worked as a Research Assistant in Clinical Pharmacology studying liver enzymes involved in detoxification. In 1995, she started working at CSIRO in the Division of Horticulture with Mark Thomas and Ian Dry and has been involved in projects applying her skills in molecular biology while learning new skills. One of the highlights in her career was being the first to genetically transform grapevine cultivars important to industry. Pat has been involved in developing genomics and generic technologies including EST and microarray analysis, making significant contributions to the NCBI EST and GEO databases. She has been involved in the discovery of genes involved in flower and fruit development and doing gene expression functional analysis of these genes in transgenic vines. Pat has also played a major role in the evaluation of new selections for industry and has developed important SNPs which now form part of routine screening of grapevine genotypes. She has developed berry and wine assays and has set up our new wine facility and has experience in wine making. Pat has since moved onto the Scion Genetics and disease Improvement project. More recently she has been working on CRISPR/Cas9 gene editing in grapevine and will be discussing some of her results in this seminar.
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